By Thom King – Food Scientist and Chief Innovation Officer

There are around 2,000 to 8,000 taste buds. Think of them as tiny flavor detectives stationed all over your tongue, eagerly waiting for delicious flavors to arrive. Each bud is home to specialized receptors, ready to spring into action as soon as they detect something flavorful.  

Taste receptors aren’t just limited to your tongue. They can also be found in other areas like your throat and even your gut. Interestingly, our taste buds can change over time, which isn’t surprising as childhood aversion to broccoli can transform into a newfound craving.

What are receptors and how do they work?
The receptors on the palate, specifically the taste receptors located on the taste buds, work through a complex process that involves the detection of chemical compounds in food and the subsequent transmission of signals to the brain. Here’s a step-by-step explanation of how these receptors function:

  • Taste Bud Structure
    • Location: Taste buds are found on the tongue, soft palate, and other areas of the oral cavity. Each taste bud contains specialized taste receptor cells.
    • Types of Taste Buds: There are different types of taste buds, each containing various types of taste receptor cells that are sensitive to different taste modalities (sweet, sour, salty, bitter, and umami).
  • Taste Receptor Activation
    • Chemical Interaction: When you eat or drink, chemicals from the food dissolve in saliva and interact with the taste receptors. Each type of receptor is sensitive to specific taste compounds:
      • Sweet: Detected primarily by T1R2 and T1R3 receptors.
      • Sour: Involves the detection of hydrogen ions (H+) through ion channels.
      • Salty: Primarily detected by epithelial sodium channels (ENaC).
      • Bitter: Detected by T2R receptors, which can recognize a wide range of bitter compounds.
      • Umami: Detected by T1R1 and T1R3 receptors, primarily recognizing amino acids like glutamate.
  • Signal Transduction
    • Receptor Binding: When a specific taste compound binds to its corresponding receptor, it activates the receptor. This activation can involve:
      • G-Protein Coupled Receptors (GPCRs): For sweet, umami, and bitter tastes, the receptors are GPCRs that initiate a signaling cascade upon activation.
      • Ion Channels: For salty and sour tastes, the receptors can act as ion channels that allow ions to flow into the taste cells.
      • Intracellular Signaling: The activation of the receptors leads to changes in the intracellular environment of the taste cells. For GPCRs, this typically involves the activation of a G-protein (like gustducin) that triggers a series of biochemical events, increasing levels of signaling molecules such as calcium ions (Ca²⁺) and cyclic AMP (cAMP).
  • Taste Cell Depolarization
    • Action Potential Generation: The increase in intracellular calcium levels or other signaling molecules causes the taste cell to depolarize. This depolarization changes the electrical potential of the cell, leading to the generation of action potentials.
  • Neurotransmitter Release
    • Signal Transmission: Once depolarized, the taste cell releases neurotransmitters (such as ATP) into the synaptic cleft, which stimulates nearby nerve fibers.
    • Nerve Fibers: The taste information is transmitted via cranial nerves (primarily the facial nerve, glossopharyngeal nerve, and vagus nerve) to the brainstem.
  • Brain Processing
    • Taste Perception: Taste signals are relayed from the brainstem to the thalamus and then to the primary gustatory cortex in the brain, where the perception of taste occurs. This is where the brain interprets the signals as specific tastes (sweet, sour, salty, bitter, umami) and integrates them with other sensory information (like smell) to create a complete flavor experience.

The relationship between sugar consumption and brain response is a fascinating area of research that highlights the intricate connection between taste, nutrition, and neurobiology. When sugar is ingested, it interacts with taste receptors on the tongue, triggering a cascade of physiological responses that ultimately influence brain activity.

Upon tasting sugar, the taste buds send signals to the brain, specifically activating the reward pathways. This process involves the release of dopamine, a neurotransmitter associated with pleasure and reward. This response not only reinforces the desire for sweet foods but also plays a significant role in our overall eating behavior.

From an evolutionary perspective, the preference for sweetness can be traced back to our ancestors, who relied on high-sugar foods for quick energy. This inherent attraction to sugar has shaped human dietary habits and preferences throughout history. However, in today’s world, where sugary foods are readily available, this natural inclination can lead to excessive consumption, potentially resulting in health issues such as obesity, diabetes, and cardiovascular disease.

Sweetness is primarily detected by taste receptors located on the taste buds of the tongue. The key receptors responsible for sensing sweet flavors are part of a family of receptors known as the T1R family, specifically T1R2 and T1R3. Here’s a closer look at how these receptors work and why they are crucial for the perception of sweetness:

Mechanism of Sweet Taste Detection

  • T1R Receptors: The T1R family includes several receptors that are sensitive to different taste modalities. T1R2 and T1R3 are specifically involved in sweet taste perception. These two receptors form a heterodimer, which is a complex of two different proteins that work together.
  • Binding of Sweet Compounds: When sweet-tasting substances (like sugars, sugar alcohols, and non-nutritive sweeteners) enter the mouth, they bind to the T1R2/T1R3 receptor complex. The binding of these sweet compounds activates the receptors, triggering a conformational change in the proteins.
  • Signal Transduction: The activation of T1R2/T1R3 initiates a biochemical cascade within the taste cells. This cascade involves the activation of a G-protein called **gustducin**, which in turn activates enzymes that increase the levels of intracellular calcium ions (Ca²⁺) and cyclic AMP (cAMP). The increase in these signaling molecules ultimately leads to the depolarization of the taste cell.
  • Neurotransmitter Release: Once the taste cell is depolarized, it releases neurotransmitters that send signals to the brain via the gustatory pathway. This neural signal is interpreted by the brain as a sweet taste.

Significance of Sweet Taste Perception

Nutritional Signaling: Sweetness is generally associated with energy-rich foods, as sugars and carbohydrates are primary sources of energy for the body. The ability to taste sweetness helps guide dietary choices, encouraging the consumption of energy-dense foods.

Evolutionary Perspective: From an evolutionary standpoint, the preference for sweet tastes may have developed as a survival mechanism. Sweetness often indicates the presence of ripe, nutritious fruits and safe sources of energy, while bitterness can signal toxicity. Thus, the ability to detect sweetness plays a crucial role in food selection and dietary behavior.

Psychological Effects: Sweet foods are often associated with pleasure and reward, activating the brain’s reward pathways. This can lead to the release of feel-good hormones such as dopamine, reinforcing the desire for sweet-tasting foods.

The T1R2 and T1R3 receptors are fundamental to the perception of sweetness on the palate. Their ability to detect a wide range of sweet compounds allows humans and other animals to enjoy and benefit from energy-rich foods.

Understanding the biology of sweet taste perception not only sheds light on basic sensory processes but also guide formulators in the development of food products, including those that seek to reduce sugar content while maintaining a desirable sweetness profile.

Positive Allosteric Modulators in Clean Label Sugar Reduction

Thaumatin is a natural protein-based sweetener derived from the katemfe fruit (Thaumatococcus daniellii), native to West Africa. It is known for its intense sweetness, approximately 2,000 to 3,000 times sweeter than sucrose, while also possessing a clean, sugar-like taste without bitter or metallic aftertastes. Thaumatin is heat-stable, making it suitable for a wide range of food and beverage applications.

The sweet taste receptor, a heterodimeric protein complex known as T1R2/T1R3, is responsible for detecting sweet compounds in the oral cavity. Thaumatin binds to a site on the T1R2 subunit of the sweet taste receptor, leading to an increase in the receptor’s sensitivity to sweet molecules and other flavors like chocolate, citrus, and flavors with warm or neutral notes. This allosteric modulation results in a synergistic enhancement of sweetness perception when thaumatin is combined with other sweetening agents such as rebaudioside M, monk fruit extract or even sugars allowing for the reduction of sugar content in products without compromising sweetness.

The synergistic effects of thaumatin with other sweetening agents or flavor enhancers is leading to the development of customized sweetener blends that mimic the taste, and mouthfeel of sugar more effectively. By combining thaumatin with other natural sweeteners and fibers, Icon Foods has been able to create sugar-reduced products that maintain a desirable sweetness profile, consumer acceptance while holding up very well in thermal processing and low ph.

Reach out to your Icon Foods representative for ThauPure thaumatin and ThauSweet DRM positive allosteric modulator samples, documentation and usage guidance. Since 1999 Icon Foods has been your reliable supply chain partner for sweeteners, fibers, sweetening systems, inclusions and sweetness modulators. Taste the Icon difference.